Application

ELISA (0.5-1 µg/ml)r>Frozen Sections (2-20 µg/ml)r>Immunoblotting (1-10 µg/ml; see comments)r>Immunoprecipitation (1-10 µg/100 µg tissue membrane)

General description

Recognizes the ~40-43 kDa GLUT-4 protein.

Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.

Immunogen

a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH

Mouse

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.

Mueckler, M. 1994. Eur. J. Biochem. 219, 713.James, D.E., et al. 1989. Nature 338, 83.James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 0.1% BSA, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)