Application

ELISA (see comments)r>Enzyme Immunoassay (≥1:5000)r>Immunoelectrophoresis (see comments)

General description

This Mouse Anti-Human IgG₁, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG₁.

Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG₁, Fc fragment.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Monospecific for human IgG₁ Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl₂ at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

250 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.

Warning

Toxicity: Standard Handling (A)