Application

Enzyme Immunoassay (1:1000)r>Immunoelectrophoresis (see comments)

General description

Immunoaffinity purified goat polyclonal antibody conjugated to β-galactosidase. Recognizes mouse IgG, heavy and light chains.

This Goat Anti-Mouse IgG, H & L Chain β-Galactosidase Conjugate is validated for use in Enzyme Immunoassay, Immunoelectrophoresis for the detection of Mouse IgG, H & L Chain.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

To achieve maximum enzyme activity, we recommend using the following substrate: r>Dissolve 1 mg 4-methylumbelliferyl-β-D-galactoside (Cat. No. 474424) in 10 ml substrate buffer (10 mM sodium phosphate, pH 7.8, with 1 mM MgCl₂) by stirring for 30 min at room temperature. Remove undissolved substrate by centrifuging or filtration. r>r>Monospecific for mouse IgG, heavy and light chains, as determined by immunoelectrophoresis against normal mouse serum. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

1 ml in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM PBS, 10 mM glycine, 10 mM MgCl₂, 1% BSA, pH 7.0.

Warning

Toxicity: Standard Handling (A)