Application

ELISA (see comments)r>Immunoblotting (1:500)r>Immunohistochemistry (see comments)r>Immunoprecipitation (see comments)r>Neutralization Studies (see comments)

General description

This Anti-α-Interferon Mouse mAb (MMHA-2) is validated for use in ELISA, Immunoblotting, IHC, IP, Neutralization Studies for the detection of α-Interferon.

Recognizes the ~19 kDa IFN-α protein.

Purified mouse monoclonal antibody. Recognizes the ~19 kDa human α-interferon (K_A ≈10⁹ M^-1).

Immunogen

α-interferon from human leukocytes

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.Rubinstein, S., et al. 1981. J. Virol. 37, 755.

Suitable for neutralization of human α-interferon. Does not recognize β-interferon or γ-interferon by immunoblotting. Effectively neutralizes human α-interferon. This antibody has also been reported to work with ELISA, immunohistochemistry, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

500 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In 18 mM Citric Acid, 165 mM Sodium Phosphate Dibasic.

Warning

Toxicity: Standard Handling (A)