Application

Enzyme Immunoassay (1:1000, see application references)

General description

This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hamilton, R.G. 1993. Clin. Chem.39, 1988.Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.Hamilton, R.G., et al. 1987. Hybridoma6, 204.Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.

Antibody should be titrated for optimal results in individual systems.r>r>Cross-reactivity by ELISA against human myeloma proteins:r>Human IgG₁: 100%r>Human IgG₂: 100%r>Human IgG₃: 100%r>Human IgG₄: 100%r>Human IgM: ﹤0.2%r>Human IgA: ﹤0.1%r>Human IgE: ﹤0.1%r>r>Cross-reactivity by ELISA against chimeric antibodies:r>Human IgG₁: 100%r>Human IgG₂: 100%r>Human IgG₃: 100%r>Human IgG₄: 100%r>Human IgM: ﹤0.01%r>Human IgA: ﹤0.01%r>Human IgE: ﹤0.01%r>r>There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.

Packaging

500 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 10 mg/ml BSA.

Warning

Toxicity: Standard Handling (A)