Analysis Note

Positive ControlRK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells

Application

Immunoblotting (4 µg/ml, colorimetric)r>Immunocytochemistry (5 µg/ml)r>Immunoprecipitation (5 µg/ml)

General description

This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.

Recognizes the Grp78 (BiP) and Grp94 proteins.

Immunogen

a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.

Kuznetsov, G., et al. 1994. J. Biol. Chem.269, 22990.Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Highly Toxic (H)