Analysis Note

Positive ControlCHO-K1 cells

Application

Immunoblotting (1 µg/ml, chemiluminescence)r>Immunocytochemistry (1:100)r>Immunofluorescence (see comments)r>Immunoprecipitation (see comments)

General description

Recognizes the ~120 kDa Lamp1 protein.

This Anti-Lamp1 Mouse mAb (LY1C6) is validated for use in Immunoblotting, Immunocytochemistry, Immunofluorescence, Immunoprecipitation for the detection of Lamp1.

Protein G purified mouse monoclonal antibody. Recognizes the ~120 kDa Lamp1 protein.

Immunogen

rat liver lysosomal membranes

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody has also been reported to work for immunofluorescence and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Kannan, K. et al. 1996. Cell Immunol.171, 10.Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.Howe et al. 1988. PNAS85, 7577.Lewis et al. 1985. J. Cell Biol.100, 1839.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS containing 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)