Application

ELISA (see comments)r>Flow Cytometry (see comments)r>Immunoblotting (1-5 µg/ml)r>Immunoprecipitation (see comments)r>Paraffin Sections (1-10 µg/ml)r>Neutralization Studies (see comments)

General description

Recognizes the ~515 kDa LRP heavy chain protein.

This Anti-LRP, Heavy Chain Mouse mAb (8G1) is validated for use in ELISA, FC, Immunoblotting, IP, Paraffin Sections, Neutralization Studies for the detection of LRP, Heavy Chain.

Purified mouse monoclonal antibody. Recognizes the ~515 kDa LRP heavy chain protein.

Immunogen

native LRP from human placenta

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Recognizes the low density heavy chain (~515 kDa) by immunoblotting under non-reducing conditions. This antibody has also been reported to work for immunoprecipitation, flow cytometry, and ELISA. Variables associated with assay conditions will dictate the proper working dilution.

Mikhailenko, I., et al. 2001. J. Biol. Chem.276, 39484.Rebeck, W.G., et al. 2001. Mol. Brain Res.87, 238.Strickland, D.K., et al. 1990. J. Biol. Chem.265, 17401.

Packaging

100 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM phosphate buffer, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)