Lumican, His#Tag«, CF, Mouse 1PC X 50UG

Code: 440319-50ug D2-231

Biochem/physiol Actions

Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 104 BCE C/...


read more

Your Price
£486.00 EACH
£583.20 inc. VAT

Biochem/physiol Actions

Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 104 BCE C/D-1b bovine cornea-derived endothelial cells.

General description

Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His Tag™ sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

D′Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266.Nikitovic, D. et al. 2008. IUBMB Life60, 818.Nikitovic, D. et al. 2008. FEBS J.275, 350.Ishiwata, T. et al. 2007. Oncol. Rep.18, 537.Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294.Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422.Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532.Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365.Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347.Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.

Packaging

50 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm filtered PBS.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 200 µg/ml with sterile PBS.

Warning

Toxicity: Standard Handling (A)

assay≥95% (SDS-PAGE)
formlyophilized
impurities≤1.0 EU/µg protein Endotoxin
manufacturer/tradenameCalbiochem®
Quality Level100
shipped inambient
storage conditionOK to freeze
storage temp.−20°C
This product has met the following criteria: