Biochem/physiol Actions
Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 104 BCE C/D-1b bovine cornea-derived endothelial cells.
General description
Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His Tag™ sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
Legal Information
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
D′Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266.Nikitovic, D. et al. 2008. IUBMB Life60, 818.Nikitovic, D. et al. 2008. FEBS J.275, 350.Ishiwata, T. et al. 2007. Oncol. Rep.18, 537.Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294.Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422.Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532.Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365.Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347.Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.
Packaging
50 µg in Glass bottle
Physical form
Lyophilized from 0.2 µm filtered PBS.
Reconstitution
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 200 µg/ml with sterile PBS.
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria: