General description

Native proMMP-1 from cultured, human rheumatoid synovial fibroblast. Corresponds to the 56 kDa/52 kDa enzyme. May contain some activated enzyme but requires APMA (Cat. No. 164610; 30-60 minutes at 37°C, just prior to use) to obtain fully activated enzyme (46 kDa/42 kDa). May also undergo autocatalysis to yield a 27 kDa/22 kDa active enzyme. Expressed by a large number of cell types. Cleaves fibrillar Type I collagen. Must be activated just prior to use.

M.W. 56,000/52,000. Note: 1 mU = 1 milliunit.

Native proMMP-1 from cultured, human rheumatoid synovial fibroblast. Corresponds to the 56 kDa/52 kDa enzyme. May contain some activated enzyme but requires activation by APMA (Cat. No. 164610) for 30 - 60 min. at 30°C just prior to use to obtain fully activated enzyme (46 kDa/42 kDa). May also undergo autocatalysis to yield a 27 kDa/22 kDa active enzyme. Expressed by a large number of cell types. Cleaves fibrillar type I collagen.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Liepinsh, E., et al. 2003. J. Biol. Chem.278, 25982.Pilcher, B.K., et al. 1997. J. Cell Biol. 137, 1445.Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.Marcy, A.I., et al. 1991. Biochemistry30, 6476.Stricklin, et al. 1983. Biochemistry22, 61.

Packaging

5 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 300 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Preparation Note

Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.

Warning

Toxicity: Standard Handling (A)