General description

Note: 1 mU = 1 milliunit.

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.

Packaging

5 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Preparation Note

Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

Warning

Toxicity: Standard Handling (A)