General description

M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.

Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Olson, M.W. et al. 2000. J. Biol. Chem.275, 2661.Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Packaging

5 µg in Plastic ampoule

Physical form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Preparation Note

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.

Warning

Toxicity: Standard Handling (A)