General description

Full-length recombinant, human MMP-13 fused to a His Tag™ sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca²⁺- and Zn²⁺-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades α₁-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2).

Note: 1 mU = 1 milliunit.

Full-length recombinant, human MMP-13 fused to a His Tag™ sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains an N-terminal propeptide which confers latency to the proenzyme, a Ca²⁺- and Zn²⁺- binding catalytic domain, a hinge region, and a C-terminal hemopexin domain. Hydrolyzes collagen type II 5-6 times faster than collagens type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Herget, J., et al. 2003. Am. J. Physiol.285, 4199.Nagase, H. 1997. Biol. Chem.378, 151.Knäuper, V., et al. 1996. J. Biol. Chem. 271, 1544.Knauper, V., et al. 1996. J. Biol. Chem.271, 17124.Pei, D., and Weiss, S.J. 1996. J. Biol. Chem.271, 9135.Knight, C.G., et al. 1992. FEBS Lett.296, 263.

Packaging

10 µg in Plastic ampoule

Physical form

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)