Analysis Note

Positive ControlRat cortex tissue extracts

Application

Immunoblotting (1:300, colorimetric) r>Immunoprecipitation (3 µg/200 µg lysate)r>

General description

Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.

Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

Immunogen

a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180Ishii, T., et al. 1993. J. Biol. Chem.268, 2836. Monyer, H., et al. 1992. Science256, 1217.

Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

15 µg in Plastic ampoule

Physical form

Lyophilized from cell culture supernatant.

Reconstitution

Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)