Application

Immunoblotting (1:1000)r>Immunohistochemistry (1:1000-1:2000)r>Immunoprecipitation (see comments)

General description

This Anti-NMDAR 2B (984-1104) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of NMDAR 2B (984-1104).

Recognizes the ~180 kDa NMDA receptor NR2B subunit.

Immunoaffinty purified rabbit polyclonal antibody. Recognizes the ~180 kDa NMDAR2B subunit of the NMDA receptor.

Immunogen

Rat Brain

a recombinant fusion protein consisting of amino acids 984-1104 of rat brain NMDAR 2B-subunit

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross-react with NMDAR2A or NMDAR2C. Please refer to the certificate of analysis for lot-specific immunoprecipitation volume. Variables associated with assay conditions will dictate the proper working dilution.

Wang, Y.H., et al. 1995. J. Neurochem.65, 176.Petralia, R.S., et al. 1994. J. Neurosci.14, 6102.Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.Monyer, H., et al. 1992. Nature256, 1217.

Packaging

10 µg in Plastic ampoule

Physical form

Lyophilized from 5 mM ammonium bicarbonate.

Reconstitution

Reconstitute in 50 µl PBS. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze thaw cycles of reconstituted solution.

Warning

Toxicity: Standard Handling (A)