Biochem/physiol Actions

ED₅₀ = 5-20 ng/ml as measured by its monocyte chemotactic activity

General description

Recombinant, human monocyte chemotactic protein-1 expressed in E. coli. A potent chemoattractant for human monocytes and mouse peritoneal macrophages. Its effects are proposed to be mediated by P₂-integrins.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Hosang, K., et al. 1994. Biochem. Biophys. Res. Commun.199, 962.Luini, W., et al. 1994. Cytokine 6, 28.

Packaging

10 µg in Plastic ampoule

Physical form

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg MCP-1.

Reconstitution

Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 1 year at -70°C.

Warning

Toxicity: Standard Handling (A)