Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

General description

Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

Note: 1 mU = 1 milliunit.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

Packaging

500 miu in Plastic ampoule

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-A-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.

Warning

Toxicity: Harmful (C)