General description

Note: 1 mU = 1 milliunit.

Recombinant, Arthrobacter ureafaciens α2-3,6,8,9-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. High concentrations of this sialidase and prolonged periods of incubation result in the additional cleavage of branched sialic acids. This enzyme is found as two active species of MW 60000 and 69000.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Means, R.E. and Desrosiers, R.C. 2000. J. Virol.74, 11181.Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.Roggentin, P., et al. 1995. Biol. Chem. Hoppe Seyler 376, 569Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.

Packaging

250 miu in Plastic ampoule

Physical form

In 20 mM Tris-HCl, 20 mM NaCl, pH 7.5.

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-methylumbelliferyl)-A-D-N-acetylneuraminic acid per min at 37°C, pH 5.0. (Note: 1 mU = 1 milliunit).

Warning

Toxicity: Harmful (C)