Analysis Note

Positive ControlMouse macrophages treated with peroxynitrite

Application

Immunoblotting (2 µg/ml)

General description

This Anti-Nitrotyrosine Mouse mAb (CC22.8C7.3) is validated for use in Immunoblotting for the detection of Nitrotyrosine.

Purified mouse monoclonal antibody. Recognizes nitrotyrosine-containing proteins.

Recognizes nitrotyrosine.

Immunogen

peroxynitrite-treated KLH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Myatt, L., et al. 1996. Hypertension28, 488.Beckman, J.S., et al. 1994. Biol. Chem. Hoppe-Seyler375, 81.

Variables associated with assay conditions will dictate the proper working dilution.

Packaging

50 µg in Plastic ampoule

Physical form

In PBS, 50% glycerol, 0.1% BSA, 0.02% sodium azide, pH 7.2.

Reconstitution

Reconstitute with H₂O to a final stock concentration of 1 mg/ml. Following reconsitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)