Biochem/physiol Actions

ED₅₀ = 2.5-12.5 ng/ml as measured in a serum-free proliferation assay using MCF7 human breast adenocarcinoma cells.

General description

Recombinant, human neuregulin 1-β1/heregulin 1-β1 extracellular domain (NRG1-beta1/HRG1-beta1 ECD) consisting of amino acids 2-246 and expressed in E. coli. All NRG1 isoforms contain an EGF-like domain (β-splice variant) that is used to directly bind to the ErbB3 or ErbB4 receptor tyrosine kinases.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Buonanno, A., and G.D Fischbach 2001. Curr. Opin. Neurobiol.11, 287.Adlkofer, K. and C. Lai 2000. Glia29, 104.Garratt, A.N. et al., 2000. BioEssays22, 987.Karey, K.P., et al. 1988. Cancer Res.48, 4083.

Packaging

50 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm filtered acetonitrile, TFA, BSA.

Reconstitution

Reconstitute 50 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Following reconstitution refrigerate (4°C) for short term-storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Warning

Toxicity: Standard Handling (A)