Biochem/physiol Actions

~200 ng immobilized osteopontin/well will mediate the adhesion of ≥25% of the 293 cells plated at 10⁵ cells/well

General description

Recombinant, mouse osteopontin fused at the C-terminus to a His Tag™ sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴ (Glu99Gly) murine myeloma cells. A secreted, highly acidic, Ca²⁺-binding, RGD-containing, phosphorylated glycoprotein. Chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. Has been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Plays a significant role in inducing granulomas of various origin. OPN is also involved in the migration of embryonic fibroblasts, macrophages, and metastatic cells.

M.W. 65,000 and 30,000 under reducing conditions.

Recombinant, mouse osteopontin fused at the C-terminus to a His Tag™ sequence and expressed in NSO-derived Leu¹⁷-Asn²⁹⁴ (Glu99Gly) murine myeloma cells. A secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein. Osteopontin is expressed in several cell types including macrophages, fibroblasts, epithelial cells, T cells and osteoclasts in response to activation by cytokines, growth factors or inflammatory mediators. Mouse OPN cDNA encodes a 294 amino acid protein that has a 16 amino acid predicted signal peptide, which is cleaved to generate 278 amino acid mature protein. The mature protein contains an integrin binding arginine-glycine-aspartic acid (RGD) sequence. Exhibits some renoprotective actions in renal injury, such as increasing tolerance to acute ischemia, inhibiting inducible nitric oxide synthase, and reducing cell peroxide levels.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Xie, Y., et al. 2001. Kidney Int.60, 1645.Chiba, S., et al. 2000. Microbiol. Immunol. 44, 319.Miyazaki, Y., et al. 1990. J. Biol. Chem. 265, 14432.

Packaging

50 µg in Plastic ampoule

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, 50 µg BSA/µg rmOPN.

Reconstitution

Reconstitute to ≥100 µg/ml with sterile PBS.

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at 4°C and for up to 3 months at -70°C. Avoid freeze/thaw cycles of stock solutions.

Warning

Toxicity: Standard Handling (A)