Analysis Note

Positive ControlUV irradiated HEK293 cells or sorbitol-treated PC12 cells

Application

Immunoblotting (1:1000)r>Immunocytochemistry (see comments)

General description

This PhosphoDetect™ Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .

Recognizes p38α, β, and γ/ERK6 phosphorylated at Thr¹⁸⁰ and Tyr¹⁸² in uv-treated HEK293 cells and sorbitol-treated PC12 cells.

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr¹⁸⁰ and Tyr¹⁸².

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Marinissen, M.J., et al. 2001. Genes Dev.15, 535.Nakagami, H., et al. 2001. Diabetes50, 1472.Wilsbacher, J.L., et al. 1999. J. Biol. Chem.274, 16988.Raingeaud, J., et al. 1995. J. Biol. Chem.270, 7420.Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA92, 10531.Han, J., et al. 1994. Science265, 808.Lee, J.C., et al. 1994. Nature372, 739.Rouse, J., et al. 1994. Cell78, 1027.

Does not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

10 tests in Plastic ampoule

Physical form

In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.3.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)