General description

Native alkaline phosphatase from human placenta.

Native alkaline phosphatase from human placenta. Homodimeric enzyme that hydrolyzes phosphate esters of primary and secondary alcohols, amines, and phenols. Useful in the hydrolysis of 5′-terminal phosphates of DNA and RNA. Active site contains a reactive serine.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Fishman, W.H., et al. 1973. Anal. Biochem.51, 368.

Packaging

100 u in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM Tris-HCl, 1 mM MgCl₂, 0.1 mM ZnCl₂, 50% glycerol, pH 7.6.

Preparation Note

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol pNPP per min at 37°C, pH 10.15.

Warning

Toxicity: Standard Handling (A)