General description

Full-length, recombinant, human phosphodiesterase 4B2 (accession no. L20971) fused at the N-terminus to a His Tag™ sequence and expressed in S. frugiperda using a baculovirus expression system. Plays a significant role in the regulation of inflammatory cell activation. PDE4B2 is a predominant isoform in neutrophils and monocytes. Supplied as a partially purified, catalytically active preparation.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.Smith, P.G., et al. 2005 Blood105, 308.Baroja, M.L., et al. 1999. J. Immunol.162, 2016.

Packaging

10 u in Plastic ampoule

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.

Warning

Toxicity: Standard Handling (A)