Analysis Note

Positive ControlAny cell lysate or tissue extract

Application

ELISA (see comments)r>Immunoblotting (1:500-1:1000, see application references)r>Frozen Sections (see application references)r>Immunocytochemistry (see application references)r>Immunoprecipitation (see comments)

General description

This PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.

Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.

Immunogen

KLH conjugated to phosphotyrosine, glycine, and alanine

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.

Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM sodium phosphate, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)