Application

ELISA (1:2000)r>Immunoblotting (1:500-1:2000)r>Immunohistochemistry (1:500)

General description

Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.

This PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.

Immunogen

KLH conjugated to phosphotyrosine, glycine, and alanine

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.

Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with ﹤150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% glycerol, 1% BSA, pH 7.4.

Warning

Toxicity: Standard Handling (A)