Application

Frozen Sections (1-2 µg/ml)r>Immunoblotting (1 µg/ml)r>Immunoprecipitation (10 µg per 200-500 µg cell lysate)r>Paraffin Sections (1-2 µg/ml, heat pre-treatment required)

General description

Recognizes the ~45-68 kDa Tau protein.

This Anti-Tau Mouse mAb (TAU-5) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tau.

Protein G purified mouse monoclonal antibody. Recognizes the ~45-68 kDa phosphorylated and unphosphorylated forms of tau.

Immunogen

Epitope: within the central region

purified bovine microtubule-associated proteins

Bovine

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Papasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA99, 1140.Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA99, 6364.

When used for formalin/paraffin embedded sections, staining is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min prior to antibody incubation. Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. Does not cross-react with other MAPS of tubulin. Recognizes both native and phosphorylated forms of tau. Variables associated with assay conditions will dictate proper working dilution.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Do not store diluted antibody without carrier protein if the concentration is ﹤50 µg/ml.

Warning

Toxicity: Standard Handling (A)