General description

Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues that function as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman′s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T-cells and human PBL blasts. Occurs as a single polypeptide chain.

Recombinant, human thioredoxin expressed in E. coli. A ubiquitous protein with two redox-active half-cysteine residues. Functions as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman's reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T cells and human PBL.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Noda, N., et al. 1997. Nippon Rinsho 55, 461.Wollman, E.E., et al. 1997. Biochem. Biophys. Res. Commun. 230, 602.Masutani, H., et al. 1996. Immunol. Lett. 54, 67.Saito, I., et al. 1996. Arthritis Rheumatism, 39, 773.Saito, N., et al. 1995. J. Immunol. 154, 3194.Buchanon, B.B., et al. 1994. Arch. Biochem. Biophys. 314, 257.

Packaging

100 µg in Plastic ampoule

Reconstitution

Reconstitute in physiological buffer containing 3 mM EDTA and 1-5 mM DTT.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Unit Definition

One unit (insulin reduction unit) is calculated by measuring the change in absorbance at 650 nm with 330 µM DTT as the reductant at 25°C, pH 6.5.

Warning

Toxicity: Standard Handling (A)