General description

Recombinant, yeast thioredoxin reductase expressed in E. coli. Yeast thioredoxin reductase is a flavoprotein that has no selenocysteine (SeCys). It catalyzes the NADPH-dependent reduction of thioredoxin as well as other endogenous and exogenous proteins. Plays an important role in many aspects of cell function, including protection against oxidative stress/injury, cell growth and transformation, and the recycling of ascorbate from its oxidized form.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Mustacich, D. and Powis, G. 2000. Biochem. J.346, 1.Wang, P.F., et al. 1998. Biochemistry37, 16378.Holmgren, A., 1979. J. Biol. Chem. 254, 9627.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from 150 mM NaCl, 20 mM phosphate buffer, pH 7.4.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for 1 month at 4°C or 3 months at -70°C.

Unit Definition

One unit is defined as the amount of enzyme that will cause an absorbance change of 1 at 412 nm using 200 µM NADPH and 5 mM DTNB, per min at 25°C, pH 7.0.

Warning

Toxicity: Standard Handling (A)