General description

Sensitive, specific detection of His Tag fusion proteins

The HIS TAG^® Monoclonal Antibody is a mouse monoclonal antibody (IgG₁) directed against the HIS TAG^® sequence encoded by many of the NOVAGEN^® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (K_d = 5 × 10^-8 to 1 × 10^-9 M) enables sensitive, specific detection of HIS TAG^® fusion proteins at antibody concentrations of 0.1-0.2 g/ml. The HIS TAG^® Monoclonal Antibody binds to N-terminal, C-terminal, and internal HIS TAG^® sequences. This antibody also detects the recombinant marker bands in the Perfect Protein﹤TMSYMBOL>﹤/TMSYMBOL> and Trail Mix﹤TMSYMBOL>﹤/TMSYMBOL> Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-g package size provides enough purified antibody for 1000 mL of working solution.* Not recommended for HRP-based detection of Trail Mix Western Markers.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

3, 100 µg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)