Analysis Note

ELISA was used to determine specificity of Anti-Mouse IgG (-chain specific)-Alkaline Phosphatase.The conjugate is specific for mouse IgG when tested against purified mouse IgA, IgG and IgM myeloma proteins.Immunoelectrophoresis (IEP) was used to establish antibody purity and identity prior to conjugation.Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.

Application

For use as secondary antibody in immuno-based assays.

Antibodies to Trypanosoma cruzi were detected in the sera of infected mice by ELISA with an alkaline phosphatase conjugated goat anti-mouse IgG chain specific. Plates were coated with T. cruzi antigen and blocked with 1% BSA/PBS prior to incubation with conjugated antibody.

Anti-Mouse IgG (γ-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1000) and western blot (1:2000). The product can also be used in immunohistochemistry (at 1:50 dilutions using formalin-fixed, paraffin-embedded sections).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Enzyme-linked immunosorbent assay (1 paper)

Biochem/physiol Actions

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallizable (fc), comprises of the H chain constant region. IgG is abundant immunoglobulin in proteins in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4. IgG antibody has enormous therapeutic potential and the Fc are is for the development of therapeutic antibody.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (γ-chain specific)-Alkaline Phosphatase antibody is specific for mouse IgG when tested against purified mouse IgA, IgG and IgM myeloma proteins.

Immunogen

Purified mouse IgG.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl₂, 50% glycerol, and 15 mM sodium azide.

Preparation Note

Affinity isolated antibody is obtained from anti-mouse IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the -chain of mouse IgG. Anti-Mouse IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.