Application

Anti-mouse IgA (α-chain specific)-peroxidase antibody can be used in modified indirect DIBA (dot- immunobinding assay) .

ELISAS were performed using HRP conjugated gaot anti-mouse IgA as the secondary antibody at a dilution of 1:10000 for a 1 hour incubation at 37 degrees to detect IgA levels in mouse fecal extracts.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Enzyme-linked immunosorbent assay (1 paper)Western Blotting (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgA antibody plays an essential role in mucosal immunity and binds with immunogens/pathogens to restrict them from entering the mucosal membrane. Anti-mouse IgA (α-chain specific)-peroxidase antibody can be used for determining the secretory IgA (sIgA) in intestinal fluid. It can also be used in direct ELISA. Goat anti-mouse IgA (α-chain specific)-peroxidase antibody reacts specifically with mouse IgA.

Immunogen

Purified mouse IgA

Packaging

1 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p 215 (1978).