Analysis Note

ControlHuman placenta lysates, rat forebrain/rostral hypothalamus.

Application

Anti-Choline Acetyltransferase Antibody has been used in:immunohistochemical stainingwestern blottingimmunocytochemistryImmunohistochemistry: A previous lot of this antibody was used at 1:100. Optimal working dilutions and protocols must be determined by end user.

Biochem/physiol Actions

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.

Immunogen

Human placental enzyme.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Research Category:NeuroscienceResearch Sub Category:Neurotransmitters & ReceptorsNeuronal & Glial Markers

Physical form

Affinity purifiedPurified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 µg of mouse brain lysates.

Specificity

Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

Target description

70/74 kDa