Application

In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Anti-Biotin antibody produced in goat has been used in multi-well plate assay, to identify desthiobiotinylating (analog of biotin) proteins. It has also been used in Colourimetric enzyme immunoassay.

Biochem/physiol Actions

Biotin serves as a cofactor for carboxylases, necessary for oxidative metabolism. Biotin absorption in small intestine is reduced on consumption of avidin rich food such as egg white. Thus, greatly decreasing its bioavailability, leading to egg white injury. Reduced levels of biotin is normally observed during pregnancy. Biotin deficiency might be congenital, with defective proteins involved in biotin homeostasis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Biotin is considered as an essential nutrient, though it is know to be secreted by some of the intestinal bacteria. It is found in liver and brain tissues in minute quantities.

Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Immunogen

Biotin-KLH conjugate

Physical form

Lyophilized from 0.01 M sodium phosphate, 15 mM sodium chloride, pH 7.2

Reconstitution

Reconstitute with 0.135 M sodium chloride.