Application

Anti-BAPTA antibody produced in rabbit is suitable for immunoblotting, immunofluorescence or immunoprecipitation at 1-10 μg/mL. The antibody is used in determining spatial distribution of BAPTA through immunocytochemistry and confocal microscopy of cortical neurons loaded with BAPTA/AM, an analog. It is also useful in determining kinetics of loading and efflux of BAPTA through ELISAs and immunocytochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BAPTA is a highly selective calcium chelator. BAPTA and its analog BAPTA/AM have been extensively used in cell physiology research for defining the mechanisms underlying inter- or intracellular calcium homeostasis and calcium dependent exocytosis. They may also be used in the study of diseases in which the regulation of excitatory neurotransmitter release and uptake has been disturbed.

Immunogen

BAPTA conjugated to KLH.

Physical form

Solution in phosphate buffered saline, pH 7.4, with 0.08% sodium azide.