Application

Anti-Beclin 1 (N-terminal) antibody produced in rabbit has been used in:immunofluorescent stainingimmunoblotting immunohistochemistryimmunoprecipitation

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

Beclin 1 (BECN1) is able to complement the autophagy deficiency of yeast lacking autophagy-related gene 6 (Atg6) and restores autophagy in human MCF7 breast carcinoma cells. Beclin 1 plays a major role in the early development of embryo. It also serves as a haplo-insufficient tumor-suppressor gene. Heterozygous Beclin 1 mutant mice have increased spontaneous tumorigenesis. Beclin 1 is monoallelically deleted in a high percentage of sporadic human breast, ovarian and prostate carcinomas and is expressed at reduced levels in those tumors. In human brains BECN1 decreases in an age-dependent fashion, possibly leading to a reduction of autophagic activity during aging, which may contribute to the accumulation of mutant Huntingtin and the age-delayed disease onset of Huntington disease.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Beclin 1 (BECN1) or GT197 is a tumor suppressor gene. It is a Bcl-2 (B-cell lymphoma 2) interacting protein and is the mammalian ortholog of yeast Atg6/Vps30. It is a 450 amino-acid protein having three domains. The gene encoding BECN1 is localized on human chromosome 17q21.

Immunogen

synthetic peptide corresponding to amino acids 29-46 of human Beclin 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.