Application

Rabbit polyclonal anti-Dog IgG (whole molecule)–FITC antibody may be used as a secondary antibody or to detect the presence of dog IgG via fluorogenic immunochemical or immunohistochemical techniques.

Anti-Dog IgG (whole molecule)-FITC antibody has been used in indirect fluorescent antibody test(IFAT)

Biochem/physiol Actions

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495nm and emits at 521nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgGis present in large quantities in the human serum and about 10-20% of the plasma proteins.IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates.It consists of four sub-classesi.eIgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γchains)and two light chains (κ or λchains)which are linked by inter-chaindisulfidebonds.The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have oneN-terminal variable domain (VL) andoneconstant domain(CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding).The antigen binds to the V regions of theantibody.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.