Application

Anti-Rabbit IgG (whole molecule)-FITC antibody produced in sheep has been used in:immunocytochemistryimmunofluorescenceimmunohistochemistry

Sheep polyclonal anti-Rabbit IgG (whole molecule)−FITC antibody may be used to detect and quantitate the level of IgG in rabbit serum and biological fluids by fluorescent techniques.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Rabbit IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in rabbit serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Sheep polyclonal anti-Rabbit IgG (whole molecule)−FITC antibody binds normal rabbit serum and rabbit IgG. It does not cross react with human IgG.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.