Analysis Note

Positive ControlMCF-7 cells

Application

Immunoblotting (1:500-1:2000)Immunoprecipitation (5-20 g/mg lysate)

General description

This Anti-Matriptase/MT-SP1 Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of Matriptase/MT-SP1.

Recognizes the ~75 kDa matriptase protein in MCF-7 cells.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~75 kDa matriptase protein.

Immunogen

a synthetic peptide corresponding to amino acids near the C-terminus of human matriptase

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Santin, A.D., et al. 2003. Cancer98, 1898.Takeuchi, T., et al. 2000. J. Biol. Chem.275, 26333.Lin, C.Y., et al. 1999. J. Biol. Chem.274, 18231.Takeuchi, T., et al. 1999. Proc. Natl. Acad. Sci. USA96, 11054.

Antibody should be titrated for optimal results in individual systems.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In Tris-citrate/phosphate buffer, pH 7.0-8.0.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Warning

Toxicity: Highly Toxic (H)