Anti-Bin1; clone 2F11

Code: mabc1190 D2-231

Application

Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin1 in 10 µg of LNCaP and C2C12 cell lysates.
Immunohistochemistry Analysis...


read more

Your Price
£345.00 EACH
£414.00 inc. VAT

Application

Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin1 in 10 µg of LNCaP and C2C12 cell lysates.
Immunohistochemistry Analysis: A representative lot detected Bin1 in Immunohistochemistry applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42).
Western Blotting Analysis: A representative lot detected Bin1 in Western Blotting applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42; Muller, A.J., et. al. (2003). Mol Cell Biol. 23(12):4295-306; Chang, M.Y., et. al. (2007). Cancer Res. 67(1):100-7).

Research CategoryApoptosis & Cancer

Detect Bin1 using this mouse monoclonal Anti-Bin1, clone 2F11 Antibody, Cat. No. MABC1190-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Bin1, clone 2F11 Antibody, Cat. No. MABC1190, is a mouse monoclonal antibody that detects Bridging integrator 1 (Bin1) protein and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: 4 µg/mL from a representative lot detected Bin1 in 10 µg of LNCaP and C2C12 cell lysates.

Immunohistochemistry Analysis: A representative lot detected Bin1 in Immunohistochemistry applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42).

Western Blotting Analysis: A representative lot detected Bin1 in Western Blotting applications (DuHadaway, J.B., et. al. (2003). J Cell Biochem. 88(3):635-42; Muller, A.J., et. al. (2003). Mol Cell Biol. 23(12):4295-306; Chang, M.Y., et. al. (2007). Cancer Res. 67(1):100-7).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myc box-dependent-interacting protein 1 (UniProt: O00499; also known as Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1) is encoded by the BIN1 (also known as AMPHL) gene (Gene ID: 274) in human. Bin1 is a ubiquitous protein that displays highest expression levels in the brain and muscle. Eleven isoforms of Bin1 have been described that are generated by alternate splicing. Isoform IIA is reported to be expressed only in the brain where it is concentrated n axon initial segments and nodes of Ranvier. It contains one BAR domain (aa 29-276) and one SH3 domain (aa 520-592). Bin1 interacts with the functionally critical Myc box regions at the N- terminus of the MYC oncoprotein and inhibits MYC-induced malignant cell transformation. Hence, it is considered as a tumor suppressor. Mutations in BIN1 gene are known to cause centronuclear myopathy that is characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles.

Immunogen

Epitope: domain of:

GST-tagged recombinant fragment corresponding to the BAR domain (aa 29-276) from the N-terminal half of human Myc box-dependent-interacting protein 1 (Bin1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry (Paraffin) in human cerebellum and human skeletal muscle tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Bin1 in human cerebellum and human skeletal muscle tissue.

Specificity

Clone 2F11 recognizes an epitope within the N-terminal BAR domain of Bin 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Stable for 1 year at 2-8°C from date of receipt.

Target description

64.70 kDa calculated.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
clone2F11, monoclonal
Gene Informationchicken ... Bin1(424228)human ... BIN1(274)mouse ... Bin1(30948)
isotypeIgG1κ, IgG1
NCBI accession no.NP_647593.1
packagingantibody small pack of 25 µg
Quality Level100
shipped inambient
species reactivitymouse, rat, human, chicken
technique(s)western blot: suitable, immunohistochemistry: suitable (paraffin)
UniProt accession no.O00499
Code
Description
Unit Size
List Price
Qty
MABC1190-25UG
Unit:25UG
List Price: £149.00
Source:List Price
ADD
This product has met the following criteria: