Application

Western Blotting Analysis: A representative lot detected BLUE in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).

Research CategorySecondary & Control Antibodies

Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-Blue antibody, Clone 2D2-F11, is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. The use of this antibody allows for visualization of marker proteins along with the protein of interest on the same blot. Anti-Blue antibody offers high specificity and does not interfere with the detection of other antibodies, In addition, it does not display reactivity with other cellular proteins, unstained marker proteins, or with proteins stained with different dyes.

Immunogen

Prestained Precision Plus Protein marker proteins.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in Pre-Stained Protein Standard.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected BLUE-stained marker protein standards.

Specificity

Anti-BLUE, clone 2D2-F11, is a mouse monoclonal antibody that detects prestained Precision Plus Protein marker proteins.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.