Application

Research Sub CategoryCytoskeleton

Anti-IL-34, clone 1D12 Antibody is an antibody against IL-34 for use in flow cytometry & immunofluorescence.

Research CategoryCell Structure

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected IL-34 in U251 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Intereukin 34, also known as IL-34 or IL34 is a cytokine that promotes the differentiation and viability of moncytes and macrophages. IL-34 stimulate the phosphorylation of MAPL1/ERK2/ERK2 and MAPK3/ERK1. IL-34 is a ligand for colony-stimulating factor CSF1R. IL-34 is predominantly expressed in spleen, and also detected in a range of other tissues including heart, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Immunogen

Purified recombinant fragment of human IL-34 expressed in E. Coli

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Quality

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected IL-34 in K562 cells.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

27 kDa calculated