Analysis Note

Positive ControlIMR5 cells

Application

Frozen Sections (1-5 µg/ml)r>Immunoblotting (1-5 µg/ml, see application references)r>Immunofluorescence (1-5 µg/ml, see application references)r>Immunoprecipitation (1-2 µg/sample, see application references)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.

Recognizes the N-myc protein and its cleavage product in IMR5 cells.

This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.

Immunogen

recombinant, human, N-myc fusion protein, expressed in E. coli

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Brondyk, W.H. 1991. Oncogene6, 1269.Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.Cole, M.D., 1986. Ann. Rev. Gen.20, 361.Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.Slamon, D.J., et al. 1986. Science232, 768.Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.Brodeur, G.M., et al. 1984. Science224, 1121.Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.Kohl, N.E., et al. 1983. Cell35, 603.Schwab, M., et al. 1983. Nature305, 245.

It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)