Analysis Note

Positive ControlReactive human fibroblasts, glucagon-producing A cells

Application

Flow Cytometry (see comments)r>Frozen Sections (1-5 µg/ml) r>Immunoprecipitation (see comments)r>

General description

Protein A purified mouse monoclonal antibody. Recognizes human fibroblast activation protein (FAP).

Recognizes fibroblast activation protein (FAP).

Anti-FAP, mouse monoclonal, clone F11-24, recognizes fibroblast activation protein in human. It is validated for use in flow cytometry, immunoprecipitation, & immunohistochemistry (frozen sections).

Immunogen

HEK293 cells transfected with an expression vector encoding full-length human FAP

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not react with resting fibroblasts of normal organ mesenchyme or parenchymal cells, except for the glucagon-producing A cells found in the pancreatic islets. This antibody has also been reported to work for flow cytometry and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)