Application

Anti-JAM-A antibody, Mouse monoclonal has been used in: western blotting staining for confocal microscopyimmunoprecipitationimmunocytochemistryflow cytometry

Biochem/physiol Actions

Junctional adhesion molecule A(JAM-A) function has been linked with regulation of cell migration/invasion, platelet adhesion, cell polarity, cell proliferation, and epithelial or endothelial barrier function. However, the mechanisms by which JAM-A mediates these processes have not been well elucidated. JAM-A functional significance is connected with inflammation, angiogenesis, hypertension, ischemia/reperfusion, and atherosclerosis. JAM-A has also been linked to breast, endometrial, renal cell carcinomas and gastric cancer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-JAM-A antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM. Junctional adhesion molecule A (JAM-A) is expressed at tight junctions of endothelial and epithelial cells as well as on a variety of hematopoietic cells. It belongs to the transmembrane IgG glycoprotein family and is also expressed in monocytes, lymphocytes, neutrophils, dendritic cells, platelets, and spermatozoa. The JAM-A or F11R gene is mapped to human chromosome 1q23.3.

Immunogen

derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM fusion protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-JAM-A antibody, Mouse monoclonal recognizes human JAM-A.

Storage and Stability

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.