Application

Anti-Rat IgG (whole molecule)-TRITC antibody has been used inconfocal microscopy immunohistochemistryimmunofluorescence

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (Fragment antigen binding) fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substratespecificity resultingin heterogenousfragments.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Binds all rat Igs

IgGis present in large quantities in the human serum. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates.It consists of four sub-classesi.eIgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains)and two light chains (κ or λchains)which are linked by inter-chaindisulfidebonds.The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have oneN-terminal variable domain (VL) andoneconstant domain(CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding).The antigen binds to the V regions of theantibody.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human samples.