Analysis Note

ControlLung

Application

Western Blot: 1:1,000 (using ECL). A single band of ~32 kD is observed. Reactivity can be completely abolished by pre-adsorption of the antibody with the complementary peptide (Catalog Number AG253). Also available are microsomal tissue preparations for control purposes (Rat spleen microsomal preparation, Catalog Number AG257).

Immunohistochemistry: 1:50-1:1,000 on human airway biopsy, human spleen, human kidney and porcine arteries.

Optimal working dilutions must be determined by the end user.

This Anti-Heme Oxygenase 1 Antibody is validated for use in IH, WB for the detection of Heme Oxygenase 1.

Research CategoryMetabolism

Research Sub CategoryToxicology & Drug Resistance

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A synthetic peptide with the amino acid sequence H-D-L-S-E-A-L-K-E-A-T-K-E-V-H-C-NH2 corresponding to amino acid residues [12-25] + Cys-NH2 of the human HO-1 protein (Yoshida et al., 1988).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Affinity Purified immunoglobulin. Liquid in PBS containing 0.01M sodium azide.

Specificity

Recognizes Heme oxygenase-1.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

32 kDa