Application

6-hydroxydopamine hydrochloride has been used:to induce Parkinson′s disease (PD) in ratsto analyse cytotoxic effect of 6-OHDA on PC12 cell lineto induce noradrenergic (NA) neuron deletion from the locus-coeruleus

Biochem/physiol Actions

Neurotoxin that destroys catecholaminergic terminals.

Caution

Hygroscopic

General description

6-Hydroxydopamine hydrochloride (6-OHDA) is a neurotoxin, commonly used to induce Parkinson′s disease (PD). 6-OHDA causes death of dopaminergic neurons in substantia nigra pars compacta progressively mimicking PD. 6-OHDA is highly oxidizable and cannot cross blood brain barrier. 6-OHDA exerts cytotoxicity by generating reactive oxygen species, initiating cellular stress and cell death. 6-OHDA leads to neuronal cell death in many in vitro models like primary neuronal culture, human neuroblastoma cell line and rat adrenal pheochromocytoma cell line, PC12.

Packaging

1 g in glass bottle

100, 500 mg in glass insert

Reconstitution

Solutions should be freshly prepared and protected from exposure to light. Solutions turn red as it oxidizes.

Dissolve in oxygen-free water containing 0.1% sodium metabisulfite or other antioxidants.