The Accuris™ Smart-Q™ 100 fluorometer is designed for the precise quantification of DNA, RNA, and protein samples. By utilising well-established methodologies for fluorescence detection, the Smart-™Q fluorometer provides higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving next generation sequencing, PCR, transfection, Western blotting, and immunoassays. The Smart-Q™ fluorometer is engineered to achieve optimal performance with Accuris’ fluorescent DNA quantification kits and assay tubes; however, it also maintains compatibility with a range of kits offered by various other suppliers.
The Smart-Q™ fluorometer, along with its accompanying assay kits, is engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Includes blue and red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements, and incorporates advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations. The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits.
- Sensitive quantitation down to 0.1pg/µL ds DNA
- Single-sample processing in 0.5mL assay tubes (SPE4016)
- Large colour touch screen for programming and operation, featuring an intuitive, icon-driven user interface
- Preprogrammed protocols allow for quick and effortless assay set-up
- Results are quickly calculated and displayed in less than 6 seconds, and saved to the instrument’s internal memory
- All data can also be exported easily to a PC using a USB flash drive.
- Built-in reagent calculators for sample preparation
- Compact and portable (11 x 6" footprint)
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