Application
Triton™ X-114 has been used:In phase separation of integral membrane proteinsIn the purification of bacterial inclusion bodiesTo study the clouding phenomenon in promethazine hydrochloride, an amphiphilic phenothiazine drugTo partition conditioned media into insoluble and soluble phases before Western blottingFor solubilization of phosphatidylinositol 4-phosphate 5-kinase from bovine brain
General description
Triton™ X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton™ X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton™ X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Packaging
1 L in glass bottle
250 mL in glass bottle
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